Homologous Recombination

wiki Def of HR: Homologous recombination is a type of genetic recombination, a process of physical rearrangement occurring between two strands of DNA. Homologous recombination involves the alignment of similar sequences, formation of a Holliday junction, and breaking and repair, known as resolution, of the DNA to produce an exchange of material between the strands. The process of homologous recombination naturally occurs in organisms and is also utilized as a molecular biology technique for introducing genetic changes into an organism.

Cloning by HR

Homologous Recombination from a Molecular Perspective



Positive-negative selection is used to enrich for ES cells containing a targeted disruption of a gene.

Transgene silencing
Site-specific gene insertion
Single copy

Homologous recombination: a basis for targeted genome optimization in crop species such as maize, Kathleen D'Halluin, Chantal Vanderstraeten, Ellen Stals, Marc Cornelissen and Rene Ruiter, Plant Biotechnology Journal 6(1):93-102 (12 Nov 2007).

Enhancing gene targeting with designed zinc finger nucleases, Bibikova, M., Beumer, K., Trautman, J.K. and Carroll, D., Science 300:764 (2003).

Lloyd, A., Plaisier, C.L., Carroll, D. and Drews, G.N. (2005) Targeted mutagenesis using zinc-finger nucleases in Arabidopsis. Proc. Natl. Acad. Sci. USA, 102, 2232–2237.

Nahon, E. and Raveh, D. (1998) Targeting a truncated Ho-endonuclease of yeast to novel DNA sites with foreign zinc fingers. Nucleic Acids Res. 26, 1233–1239.

Urnov, F., Miller, J., Lee, Y.-L., Beausejour, C., Rock, J., Augustus, S., Jamieson, A., Porteus, M., Gregory, P. and Holmes, M. (2005) Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature, 435, 646–651.

Wright, D., Townsend, J., Winfrey, R., Irwin, P., Rajagopal, J., Lonosky, P., Hall, B., Jondle, M. and Voytas, D. (2005) High-frequency homologous recombination in plants mediated by zinc-finger nucleases. Plant J. 44, 693–705.